Turf War battles have become popular among young Inklings. They flock to Inkopolis from the countryside to battle it out from dawn till dusk. Around this time, Callie and Marie make their debut as the Squid Sisters. Starting with City of Color, they release a series of singles. Calamari Inkantation, their fourth, becomes a huge hit.
The duo was originally known for having won Calamari County's first annual Youth Folk-Singing Contest. Meanwhile, in Octo Valley, there's a power shortage where the Octarians live. To supplement their limited supply, the Octarian army kidnaps the Great Zapfish and smaller Zapfish from Inkopolis.
In response, Cap'n Cuttlefish reforms the Squidbeak Splatoon with his granddaughters Callie and Marie, who become Agent 1 and Agent 2.
They are formally called the New Squidbeak Splatoon.